Inverted Fluorescence Microscope LX404IIM

Description

Inverted Fluorescence Microscope LX404IIM is developed with a phase contrast system and a hinged trinocular observation viewing head. Equipped with high eye point, large field, flat eyepiece HWF 10x/25 mm and long working distance Infinity Plan Semi-apochromatic phase contrast objectives. This fluorescence microscope offers high-quality performance. It gives detailed fluorescent images of the specimen studied.

Specifications

Viewing head
Trinocular head, 45° inclined tubes with 100 - 0/20 - 80/0 - 100 beam splitters
Interpupillary distance
47 - 78 mm, Diopter adjustment on both eyepieces
Eyepiece
HWF 10x/25 mm Eyepieces
Nosepiece
Motorized sextuple revolving nosepiece, optionally compatible with DIC prisms for 10x, 20x, and 40x objectives
Side port
Motorized slide port, life - 100/0, eye 100, right - 80.20
Objectives
All are anti-fungal, anti-reflection coated optics for optimal light transmission.
Infinity plan semi-apochromatic phase contrast objectives (N-iPLFN PH) 4x/0.13 NA, WD 17.78 mm; requires phase annuli
High numerical infinity semi-apochromatic LWD plan phase objectives enable the subcellular structures
Infinity plan semi-apochromatic phase contrast objectives (N-iPLFN PH) 10x/0.30 NA, WD 10.2 mm; cover glass correction 1.2 mm
Infinity plan semi-apochromatic phase contrast objectives (N-iPLFN PH) 20x/0.45 NA, WD adjustable 7.5 - 8.8 mm (collar ring); cover glass correction 1.2 mm
Infinity plan semi-apochromatic phase contrast objectives (N-iPLFN PH) 40x/0.60 NA, WD adjustable 3 - 4.4 mm (collar ring); cover glass correction 1.2 mm
Stage
Three-layer motorized stage 390 x 230 mm, equipped with a coaxial mechanical 114 x 76 mm X - Y Stage, equipped with:
Metal insert for ø 38 mm cell culture dish
Metal insert for ø 54 mm cell culture dish or 76 x 26 mm slides
Metal insert for multiwell plates (127.76 x 85.48 mm)
Metal insert for teraski plates (83.3 x 58.0 mm)
Condenser
Motorized rotating condenser NA 0.55, WD 26 mm with iris diaphragm; phase annuli for 10x, 20x, 40x, 60x; DIC prism compatibility; 3 filter holders (Ø38 mm); tilting arm for large samples
Display
LCD screen display, the front-positioned, back-illuminated LCD, filter cube, and light intensity
Foucing
Motorized focusing with electronic limits, auto lens lowering, electronic parfocal; stroke range: +8 mm/-2 mm; total travel 10 mm approx.
Lens changer
Motorized Electric lens changer with 1.5x lens enables intermediate magnifications; Switching from 20x to 30x, 40x to 60x and 60x to 90x magnification done without refocusing
Illumination
Transmitted 10 W LED illumination with adjustable intensity
Fluorescence kit
6 Position fluorescence unit with UV shield; 4-color high-power LED source; filters:
Blue: EX 460 - 495 nm, DM 505, EM 510 nm
Green: EX 510 - 550 nm, DM 570, EM 575 nm
UV: EX 330 - 385 nm, DM 400, EM 420 nm
C mount
1 x C mount
Digital camera
CMOS camera; USB 3.0; 2-stage Peltier cooling to -42°C below ambient; 20 MP (IMX183, 1 sensor 13.06 x 8.76 mm); pixel size 2.4 x 2.4 µm; suitable for low-light and fluorescence imaging
Power supply
100 - 240 V
Packing dimension and gross weight
600 x 600 x 550 mm; 32 Kg
620 x 570 x 470 mm; 20 Kg
Features
  • Ergonomic trinocular head with variable beam splitting for flexible imaging
  • Wide-field HWF 10x eyepieces with diopter adjustment
  • Motorized sextuple nosepiece with optional DIC compatibility
  • Infinity plan semi-apochromatic PH objectives with high NA and long WD for subcellular resolution
  • Anti-fungal, anti-reflection coated optics for high transmission efficiency
  • Motorized XY stage compatible with slides, dishes, multiwell and Terasaki plates
  • Motorized condenser with phase/DIC support and tilt-back mechanism
  • Equipped with a magnification changer for seamless intermediate zoom without refocus
  • High-intensity LED transmitted illumination with adjustable output
  • Integrated LCD status display for system parameters
  • Multi-channel fluorescence system with high-power LED excitation
  • Cooled CMOS scientific camera for high-sensitivity low-light imaging
Applications
  • Suitable for live cell imaging, fluorescence observation, and time-lapse studies in biological research. Widely used in cell culture analysis, molecular biology, and biomedical diagnostics. Ideal for advanced applications including subcellular imaging, drug research, and fluorescence-based assays.
Standard Accessories
  • Fluorescence filter wheel (Without filters)
  • Blue and green Ø 45 mm interference filters
  • Alignment telescope
  • Power cord